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NOTE:
If no value is entered into the Concentration column, the default values set in the
Edit: Fluid Defaults pane of the Options dialog box will be used for those cells.
3.
Type the plate assay volume and the DMSO limit for the first plate into the Assay Properties
box. If more than one plate is in a configuration, they may each have different assay volumes
and DMSO limits.
NOTE:
If the fluids to be dispensed into a well exceed the DMSO limit, a warning will be
generated and the well pop-up data displayed for that well will appear in red; however, the
Dispenser will dispense whatever concentration/volume you have specified after you have
been warned.
4.
In order to program a fluid into wells, the Edit check box in the Fluids List for that fluid must be
selected. When the Edit check box is selected, that fluid can be added or removed from wells,
and is visible in the well plate image. Fluids with the Edit box not checked cannot be added or
removed from wells, and if that fluid was previously programmed into wells, it will remain in the
well plate layout and will be dispensed during the Go process, but will not be shown in the well
plate image. Clear the Edit check box if you want to simplify the view or copy and paste only
some fluids in wells where multiple fluids are being dispensed. The effect of unchecking an
Edit check box is shown in
5.
To set a fluid or fluids to be non-dispensed, select the NoDispense check box in the Fluids
List. This may be useful when you want to maintain information about nondispensed fluids in
the output report such as for manually or robotically pipetted controls, or for blanks.
6.
To program more than one well plate to be run with the same fluids list, select Add Plate. To
remove the current plate from the current configuration, select Remove Plate.
The Total Volume column values cannot be changed. The total volume is the sum of the volume of
a fluid in all of the wells of the plate, also known as the dispense volume of the fluid. This total
volume is less than the volume you will be asked to load in the DispenseHead Cassette due to the
amount of fluid retained in the DispenseHead after dispense, also known as the dead volume.
5-2 Edit the Fluids List
In addition to typing directly into the Fluids List table as described in
Fluids List can also be edited by clicking Edit and then Fluids List.
1.
Select one or more fluids you would like to edit by clicking on the row number (in the left-most
column in the Fluids List). To select multiple fluids, hold down the CTRL key while clicking on
the row number.
2.
Click Edit and then Fluids List.
○
Select Enable All for Editing or Disable All for Editing to change all fluids to be shown or
not shown in the well plate image, and able to be added or not to the plate layout.
○
Select Move to Top, Move Up, Move Down, or Move to Bottom to change the order in
which fluids are dispensed into the well plate.
○
Select Delete Fluid to remove the selected fluid from the table.
○
Select Copy, Paste, or Insert Fluid to copy, paste, or insert a fluid into the table.
○
Select Clear Fluids List to remove all fluids from the Fluids List.
NOTE:
These options are also available by right-clicking on selected fluids in the Fluids List.
Figure
5-4.
HP D300 Digital Dispenser
―Create the fluids
table‖, the
32
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